Refine your search
Collections
Year
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Widjiati,
- Placental Cells Number and Caspase-9 Expression in White Rat (Rattus norvegicus) Apoptosis Exposed with Carbon Black
Abstract Views :170 |
PDF Views:0
Authors
Affiliations
1 Department of Anatomy Veterinary, University of Airlangga, Surabaya, ID
2 Department of Animal Reproduction, Faculty of Veterinary Medicine, Brawijaya University, Malang, East Java, ID
1 Department of Anatomy Veterinary, University of Airlangga, Surabaya, ID
2 Department of Animal Reproduction, Faculty of Veterinary Medicine, Brawijaya University, Malang, East Java, ID
Source
Research Journal of Pharmacy and Technology, Vol 12, No 4 (2019), Pagination: 1935-1942Abstract
Pollutants include particulate matter suspended referred to as Particulate Matter (PM). Soot is one of the components of PM that are known to be cytotoxic and causing the occurrence of inflammation in cardiovascular and respiratory systems and can pass through the placenta barier may affect to the fetus. Effect by pollutants depends on the magnitude of exposure and length of time of exposure. This research aims to identify the Caspase-9 as an indicator of apoptosis on placenta white rats pregnant (Rattus norvegicus) is being exposed carbon black with immunohistokimia techniques and cell number tropoblas due to exposure to carbon black. This research begins with a fecundate female rats and exposure with carbon black dosage 534 mg/m3 and 1064 mg/m3. The next step is the observation and examination of the expression of Caspase-9 by immunohistokimia staining of white rat placenta that had being with carbon black. The design of this research using design random group who were conceived to prove the existence of expression caspase-9 as an indicator that mediate apoptosis in white rats pregnant is being exposed carbon black. The number of cells tropoblast analyzed by using analysis of variant (ANOVA) and the difference between the control group with treatment in a test using LSD test. The result of expression Caspase-9 in pregnancy 6-17 day with exposure to carbon black doses 1064 mg/m3 shows the results of significantly. It showed that the age of pregnancy affect the expression Caspase-9. On the outcome of research on pregnancy day to 6-11 and day 6-17 show a significant difference when a compare to control both on a cell cytotrophoblast and synsitiotrophoblast. Conclusion this research is that influence exposure carbon black on the placenta rats white (Rattus norvegicus) with long exposure to 6-11 the during the pregnancy, doses 534 mg/m3 and 1064 mg/m3, increase expression protein Caspase-9 as an indicator mediate apoptosis along with long exposure to and increased doses and decrease the total cell tropoblast placenta due to exposure to carbon black along with increasing long and dosage of exposure.Keywords
Carbon Black, Caspase-9, Tropoblast, Placenta Cell.
References
- Hickman AJ. Methodology for Calculating Transport Emissions and Energy Consumption. Transport Research Laboratory. 1999.
- Dejmek J, Selevan SG, Benes I, Solanský I, Srám RJ. Fetal growth and maternal exposure to particulate matter during pregnancy. Environ Health Perspect. 1999;107(6):475-80.
- Dachlan EG, Widjiati, and B. Santoso. Pengaruh Paparan Partikulat Jelaga Terhadap Peningkatan Lipid Peroksidase, Kejadian Apoptosis Plasenta Dan Luaran Kebuntingan Pada Mekanisme Molekuler Gangguan Kebuntingan Tikus (Rattus novergicus). Universitas Airlangga. 2011.
- Ishioka S, Ezaka Y, Umemura K, Hayashi T, Endo T, Saito T. Proteomic analysis of mechanisms of hypoxia-induced apoptosis in trophoblastic cells. Int J Med Sci 2007; 4(1): 36-44.
- Setyaningsih, E. Konsumsi Oksigen Tikus Putih (Rattus norvegicus L.) Strain Wistar yang Terdedah Asap Rokok-Berfilter dan Tanpa Filter. Prosiding Seminar Nasional Pendidikan Sains “Pengembangan Model dan Perangkat Pembelajaran untuk Meningkatkan Kemampuan Berpikir Tingkat Tinggi” 2015; 573-82.
- Tursinawati Y, Yazid N, Purnawati FW. Gambaran histopatologi ventrikel kiri tikus yang diberi paparan rokok elektrik (ENDS) dan konvensional. Qanun Medika 2017; 1(2): 87-93.
- Hougaard KS, Jansen KA, Nordly P, Taxvig C, Vogel U, Saber AT, and Wallin H. Effects of prenatal exposure to diesel exhaust particles on postnatal development, behavior, genotoxicity and inflammation in mice. Particle and Fibre Toxicology 2008; 5:3
- Nowak M, Madej JA and Dziegiel P. Expression of Breast Cancer Resistance Protein (BCRP-1) in Canine Mammary Adenocarcinomas and Adenomas. in vivo 2009; 23: 705-710
- Donaldson K, Tran L, Jimenez LA, Duffin R, Newby DE, Mills N, et al. Combustion derived nanoparticles: A Review of their toxicology following inhalation exposure. Particle and fibre toxicology 2005; 2:10.
- IARC. IARC monographs on the evaluation of carcinogenic risk of chemicals to humans. Carbon black, Titanium Dioxide, and Talc 2010. vol 93. Lyon: World Health Organization, International Agency for Research on Cancer, IARC Press.
- Hougaard KS, Fadeel B, Gulumian M, Kagan VE, Savolainen K. Developmental toxicity of engineered nanoparticles. In: Gupta RC, editor. Reproductive and Developmental Toxicology. Academic Press, Amsterdam. 2011. pp 269-290
- Driscoll KE, Costa DL, Hatch G, Henderson R, Oberdörster G, Salem H, et al. Intratracheal instillation as an exposure technique for the evaluation of respiratory tract toxicity: Uses and limitations. Toxicol Sci 2000; 55:24–35.
- Jackson P, Hougaard KS, Boisen AMZ, Jacobsen NR, Jensen KA, Møller P, et al. Pulmonary exposure to carbon black by inhalation or instillation in pregnant mice: Effects on liver DNA strand breaks in dams and offspring, Nanotoxicology 2012. 6:5, 486-500.